RBC SSP-PCR
RBC-Ready Gene
Red Blood Cell-Ready Gene is inno-train’s product line for analysis of erythrocyte blood groups based on the SSP-PCR method.
Therefore these diagnostic systems represent the ideal complement to serolgoical blood group typing. The modular kit design offers an extensive choice for a gradual typing system depending on your problem.
The evaluation is performed by standard agarose gel electrophoresis. As an internal PCR control each tube contains primers for amplification of the Human Growth Hormone (HGH). If no specific product is present after PCR, the amplificate of this positive control must be clearly detectable.
Advantages of the Ready Gene Systems
high flexibility due to modular product concept
easy and fast test performance
safety with polytransfused patients
clarification of questionable RHD/RHCE
negative controls included
coloured primer mixes for better visibility
CE certified
Downloads
Flyer RBC-Ready Gene
AB0 Genotypisierung
Patients with weak AB0 expression or with a questionable pattern of their isoagglutinins can be retyped with RBC-Ready Gene AB0/AB0 Subtype up to very weakly expressed antigens. The flexible format of RBC-Ready Gene allows the performance of both kits in parallel, or only the subtype depending on your requirements.
Genotyping of Rare Blood Groups
In many cases antisera against rare blood groups are not available or difficult to identify and that can be directed against rare blood groups. RBC-Ready Gene Rare ID detects rare blood group alleles without a doubt and in an economical way.
In times of a globalized world the demand for blood units with rare blood group alleles is increasing. Our screening concepts for rare blood group alleles support you in fining the suitable blood unit. You have the choice: the RBC-Ready Gene Rare Screen system screens with only one reaction for five different blood group alleles. The RBC-Ready Gene 4-Screen Sseaches in four reaction of seven different rare blood group alleles in parallel with the confirmation of D positivity or D negativity. Further identification of the detected rare allele after such a screening test can be completed with the RBC-Ready Gene Rare ID system.
Genotyping of the KELL, KIDD, DUFFY and MNS systems
Different hemolytic diseases e. g. sickle cell anemia or thalassemia, but also complex courses of diseases following multiple operations due to accidents or chronic diseases require continuous blood transfusions. As a result the recipients have a mixture of blood that makes blood group detection difficult, expecially of the blood groups Kell, Kidd, Duffy and MNS. If further transfusions are necessary molecular blood group detection gives clear result because the colume of donor DNA in the transfusedblood units dies not influence the test.
RBC-Ready Gene also gives you safety in typing patients who have generated allo- or autoanitbodies and/or show a positive DAT in serological typing.
Beside the standard RBC-Ready Gene MNS and RBC-Ready Gene KKD test systems we offer the RBC-Ready Gene KELplus Kit for detection of further Kell alleles, as well as the RBC-Ready Gene JKplusFY for detection of further Kidd alleles in combination with Duffy detection.
RHD Genotyping
For clarification of serologically weak D typings in patients and donors inno-train’s system RBC-Ready Gene CDE and RBC-Ready Gene D weak can be used individually or in combination. Ambiguous samples are tested specifically for D categroies, D partial and D weak and can therefor be clearly characterized.
Molecular retyping of samples are serologically D negative with a C or E antigen show a D positivity (DEL, D weak, D variant) in rare cases.
The RBC-Ready Gene D AddOn systems detects additional RHD sequences and further D negative alleles, which are not caused by a deletion of the whole RHD gene, i. e. DELs, RHDpsi, d(C)es, D-CE(2-9)-D or W16X.
The detection of these alleles represents important additional information for correct zygosity typing. All zygosities with known D negative alleles are detected safely by the combination of both systems.
Further RHCE Analysis: RBC-Ready Gene RHCE variants
RBC-Ready Gene CDE
It is also applicable for analyzing anomalous serological typings, e. g. unexpected RH antibodies. Furthermore it can be used for a targeted testing of donors/patients for altered RHCE variant expressions.
RBC-Ready Gene vERYfy: The combined solution
Samples from polytransfused patients or from patients producing allo-/auto-antibodies mostly requre a clear molecular clarification. The RBC-Ready Gene vERYfy system offers a combined solution for the parallel analysis of the blood group systems RHD, RHCE, MNS, Kell, Kidd, Duffy and Dombrock.
Products:
RBC-Ready Gene (SSP) - Genomic Blood Group Detection
Item number | Name | Test / Plate |
---|---|---|
001 010 012 | RBC-Ready Gene AB0 | 12 |
001 012 012 | RBC-Ready Gene AB0 Subtype | 12 |
001 020 012 | RBC-Ready Gene CDE | 12 |
001 022 012 | RBC-Ready Gene D weak | 12 |
001 040 012 | RBC-Ready Gene KKD | 12 |
001 042 012 | RBC-Ready Gene KELplus | 12 |
001 044 012 | RBC-Ready Gene JKplus Fy | 12 |
001 050 012 | RBC-Ready Gene MNS | 12 |
001 061 012 | RBC-Ready Gene ZygoFast | 12 |
001 062 012 | RBC-Ready Gene D AddOn | 12 |
001 063 024 | Combi Kit ZygoFast + D Add On | |
001 070 012 | RBC-Ready Gene Rare ID | 12 |
001 072 096 | RBC-Ready Gene Rare Screen | 96 |
001 075 024 | RBC-Ready Gene 4-Screen | 24 |
001 076 012 | RBC-Ready Gene vERYfy | 12 |
001 077 012 | RBC-Ready Gene RHCE variants | 12 |
001 080 024 | RBC-Ready Gene D weak Screen | 24 |