HLA-Ready Plate

HLA-Ready Plate

HLA-Ready Plate - Microlymphocytotoxicitytest

  • Thaw Ready Plates 10 - 15 min. before use
  • Dispense 1 µl (2000 - 3000) lymphoytes to each well in the tray
  • Incubate at room temperature for 30 minutes (DR/DQ: 60 min.)
  • Thaw frozen rabbit complement of reconstitute lyphilized complement
  • Add 5 - 6 µl rabbit complement to each well and incubate at room temperature for 60 min.
    (DR/DQ: 120 min.)
  • Add 3 - 4 µl easin (5 %), wait 5 minutes, add 5 - 6 µl formaldehyde (37 %) for fixation
  • Cover with glass slides and read the Ready Plates with the inverted phase microscope
The percentage of positive cells can be reported in scores
  Score 1 0 - 10 % negative reaction  
  Score 2 11 - 20 % borderline negative reaction  
  Score 4 21 - 50 % borderline positive reaction  
  Score 6 51 - 80 % positive reaction  
  Score 8 81 - 100 % strong positive reaction  
  Score 0 no cells  
Art. No. Name Test / Plate
RUO Not for use in diagnostic procedures - USA and Canada
002 011 144 HLA-Ready Plate ABC 144 5
002 010 144 HLA-Ready Plate AB 144 5
002 011 120 HLA-Ready Plate ABC 120 5
002 010 120 HLA-Ready Plate AB 120 5
002 012 072 HLA-Ready Plate ABC 72 10
002 014 072 HLA-Ready Plate AB 72 10
002 013 072 HLA-Ready Plate DR 72 10
002 021 001 HLA-Ready Plate B27 1 10
002 022 002 HLA-Ready Plate B27 2 20
002 023 005 HLA-Ready Plate B27 5 50
002 025 005 HLA-Ready Plate ABC Complement 5
Art. No. Name Test / Plate
RUO Not for use in diagnostic procedures - USA and Canada
002 041 600 Lymphodex 6 x 100 ml
002 041 500 Lymphodex 500 ml
002 042 600 Hanks Solution 6 x 100 ml
002 042 500 Hanks Solution 500 ml
002 043 500 RPMI-1640 500 ml
002 044 005 Rabbit Complement 5 x 1 ml
002 044 005L Lyophilized Rabbit Complement 5 x 1 ml
002 045 001 HLA Control positive 0.5 ml
002 045 002 HLA Control negative 0.5 ml
002 047 500 Lymphofix 500 ml

Flyer HLA-Ready Plate

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