FluoGene^® is a unique method for molecular HLA-, RBCand HPA typing combining all advantages of the SSPPCR with the speed of endpoint fluorescence detection. The analysis is based on a specifically modified TaqMan^® probe system by inno-train. Up to four fluorophores are detected by the FluoVista^® analyzer and an integrated evaluation is realized by the FluoGene^® software. Plate-specific data can be easily read from the barcoded FluoGene^® PCR plate and sample-ID can be scanned and automatically transferred into the FluoGene^® Software. A fully automated workflow can be realized by the use of our small pipetting unit PiU1. Just scan the barcode of the FluoGene^® PCR plate for an automated protocol selection („Scan & Go“).
FluoGene^® is a self-contained system comprising the ready-to-use kits, the detection device and the software solution displaying the typing results immediately after the evaluation read. For the PCR set-up simply add your diluted sample DNA to the ready-to-use FluoMix and dispense 15 μl per well over the PCR plate. This manual step can be performed automated by the pipetting unit PiU1. Afterwards the FluoGene^® plates are sealed with an adhesive optical foil. The subsequent pre- and postreads are generated in a closed environment. Therefore any risk of a post-PCR contamination gets eliminated. The FluoGene^® Software supplies the immediate display of typing results, as well as a fully-automated data management.

DNA concentration can be rapidly measured in 96 well format using fluorescent dyes. This enables a very fast, accurate, reasonable priced and automated process - measurement and typing of your samples in one system! The system is validated with QuantiFluor^® by Promega, a fluorescence dye intercalating specifically in double-stranded DNA for specific and sensitive quantification. Just add your sample and dye to a 96 well plate - that`s all.

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