HLA-SuBiTo

HLA-SuBiTo - The Sequencing

Same simple and uniform set up for all HLA-SuBiTo kits

  • To reduce ambiguities two amplifications are carried out in parallel in order to separate many heterozygous allele combinations in the first step
  • Gel Electrophoresis
  • Purification of the PCR products using ExoSAP-ITTM
  • 6 sequence reactions per HLA Class I Locus sequence
  • 3-5 sequence reactions per HLA Class II Locus sequence
  • The combination of forward and reverse reactions takes place according to positive PCR amplification
  • Precipitation with IT-Prec-Buffer
  • Loading of the capillary sequencer
  • Analysis with HLA-HiType software

New kit improvements save you 3 hours assay time

  • New chemistry on G1/G2 mixes enable shorter PCR profile for first PCR - takes now only 1 h 45 min
  • Shorter protocol for Cycle Sequencing PCR takes now only 1 h 10 min
  • Shorter precipitation protocol saves 30 minutes

Complete kit contains

  • All components for amplification including IT-Taq Polymerase
  • Exo-SAP-ITTM for purification of the PCR products
  • Gene locus and exon-specific sequencing mixes incl. BigDye® chemicals
  • IT-Prec Buffer for precipitation of the sequencing products
  • Second Stage sequencing mixes for resolution of common ambiguities are directly included in the kit
Art. No. Name Test / Plate
002 090 025 HLA-SuBiTo A 25
002 090 025/1 HLA-SuBiTo A without BDT's 25
002 091 025 HLA-SuBiTo B 25
002 091 025/1 HLA-SuBiTo B without BDT's 25
002 092 025 HLA-SuBiTo C 25
002 092 025/1 HLA-SuBiTo C without BDT's 25
002 093 025 HLA-SuBiTo DRB1 25
002 093 025/1 HLA-SuBiTo DRB1 without BDT's 25
002 094 025 HLA-SuBiTo DQB1 25
002 094 025/1 HLA-SuBiTo DQB1 without BDT's 25
002 095 025 HLA-SuBiTo DPB1 25
002 095 025/1 HLA-SuBiTo DPB1 without BDT's 25
002 096 025 HLA-SuBiTo ABCDRDQ 25
002 096 025/1 HLA-SuBiTo ABCDRDQ without BDT's 25

Flyer HLA-SuBiTo


Second Level Sequencing

On the strength of the HLA-SuBiTo two amplification strategy, ambiguous heterozygotes due to cis-trans ambiguities are reduced severely. However in case the groups could not be separated, possible ambiguities can be resolved with the ready-to-use Second Level Sequencing Mixes using the existing PCR product of the standard kit.

There are a total of 16 different Second Level Sequencing Mixes available for HLA class I, 6 different Mixes for HLA-DRB1 and HLA-DQB1, and 4 mixes for HLA-DPB1.

The powerful HLA-HiType Software proposes in the case of ambiguities the ideal Second Level Sequencing Mix for resolution.

Art. No. Name Test / Plate
002 290 001 HLA-SuBiTo A Seq2 No1 10
002 290 002 HLA-SuBiTo A Seq2 No2 10
002 290 003 HLA-SuBiTo A Seq2 No3 10
002 290 004 HLA-SuBiTo A Seq2 No4 10
002 290 005 HLA-SuBiTo A Seq2 No5 10
002 290 006 HLA-SuBiTo A Seq2 No6 10
002 290 013 HLA-SuBiTo A Seq2 No13 10
002 290 020 HLA-SuBiTo A Seq2 No20 10
002 290 025 HLA-SuBiTo A Seq2 No25 10
002 290 027 HLA-SuBiTo A Seq2 No27 10
Art. No. Name Test / Plate
002 091 002 HLA-SuBiTo B Seq2 No. 2 10
002 091 006 HLA-SuBiTo B Seq2 No. 6 10
002 091 007 HLA-SuBiTo B Seq2 No. 7 10
002 091 011 HLA-SuBiTo B Seq2 No. 11 10
002 091 013 HLA-SuBiTo B Seq2 No. 13 10
002 091 015 HLA-SuBiTo B Seq2 No. 15 10
002 091 018 HLA-SuBiTo B Seq2 No. 18 10
002 091 021 HLA-SuBiTo B Seq2 No. 21 10
002 091 029 HLA-SuBiTo B Seq2 No. 29 10
002 091 030 HLA-SuBiTo B Seq2 No. 30 10
002 091 031 HLA-SuBiTo B Seq2 No. 31 10
Art. No. Name Test / Plate
002 092 005 HLA-SuBiTo C Seq2 No. 5 10
002 092 006 HLA-SuBiTo C Seq2 No. 6 10
002 092 008 HLA-SuBiTo C Seq2 No. 8 10
002 092 009 HLA-SuBiTo C Seq2 No. 9 10
002 092 010 HLA-SuBiTo C Seq2 No. 10 10
002 092 013 HLA-SuBiTo C Seq2 No. 13 10
002 092 016 HLA-SuBiTo C Seq2 No. 16 10
002 092 017 HLA-SuBiTo C Seq2 No. 17 10
002 092 023 HLA-SuBiTo C Seq2 No. 23 10
002 092 225 HLA-SuBiTo C Seq2 No. 25 10
002 092 028 HLA-SuBiTo C Seq2 No. 28 10
002 092 029 HLA-SuBiTo C Seq2 No. 29 10
Art. No. Name Test / Plate
002 295 001 HLA-SuBiTo DPB1 Seq2 No1 10
002 295 002 HLA-SuBiTo DPB1 Seq2 No2 10
002 295 003 HLA-SuBiTo DPB1 Seq2 No3 10
002 295 004 HLA-SuBiTo DPB1 Seq2 No4 10
Art. No. Name Test / Plate
002 294 001 HLA-SuBiTo DQB1 Seq2 No1 10
002 294 002 HLASuBiTo DQB1 Seq2 No2 10
002 294 003 HLA-SuBiTo DQB1 Seq2 No3 10
002 294 004 HLA-SuBiTo DQB1 Seq2 No4 10
002 294 005 HLA-SuBiTo DQB1 Seq2 No5 10
002 294 006 HLA-SuBiTo DQB1 Seq2 No6 10
002 294 007 HLA-SuBiTo DQB1 Seq2 No7 10
002 294 009 HLA-SuBiTo DQB1 Seq2 No9 10

Analysis with HLA-HiType Software

  • Integration of new nomenclature and automated translation in P- and G- groups as well as new NMDP-Codes
  • Fully automated data management, no exhausting selection of *.ab1 Files necessary
  • Automated recognition and dynamic display of hemi- and heterozygous sequences
  • Several manual and automated editing possibilities
  • Diverse alignment options, combined control of electropherogram and alignment
  • High safety due to a defined 4 eyes principle (technical and medical validation)
  • Complete history with a traceability of each step 

Contact Form

* obligatory

Call

+49 (0) 6173 607930