Certified according to
DIN EN ISO 13485:2016

HLA Serology

HLA-Ready Plate

Your contact
Dr. Jan Konietzka
Phone: +49 6173 6079 30
E-mail: j.konietzka@inno-train.de
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Thaw HLA-Ready Plate plates 10-15 min. before use

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Dispense 1 µl (2000 - 3000) lymphoytes to each well in the tray

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Incubate at room temperature for 30 minutes

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Thaw frozen rabbit complement of reconstitute lyphilized complement

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Add 5 - 6 µl rabbit complement to each well and incubate at room temperature for 60 min

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Add 3 - 4 µl easin (5 %), wait 5 minutes, add 5 - 6 µl formaldehyde (37 %) for fixation

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Cover with glass slides and read the Ready Plates with the inverted phase microscope

Downloads

Flyer HLA-Ready Plate

The percentage of positive cells can be reported in scores

Score 1

0 – 10%

negative reaction
Score 2
11 – 20%
borderline negative reaction

Score 4

21 – 50%
borderline positive reaction

Score 6

51 – 80%

positive reaction

Score 8

81 – 100%

strong positive reaction
Score 0
no cells

Products:

HLA Ready Plate Kits
Item numberNameTest / Plate
002 011 144HLA-Ready Plate ABC 1445
002 011 120HLA-Ready Plate ABC 1205
002 012 072HLA-Ready Gene ABC 7210
002 021 001HLA-Ready Plate B27 110
002 025 005HLA-Ready Plate ABC Complement5
Media and Accessories
Item numberNameTest / Plate
002 041 600Lymphodex6 x 100 ml
002 041 500Lymphodex500 ml
002 042 600Hanks Solution6 x 100 ml
002 042 500Hanks Solution500 ml
002 043 500RPMI-1640500 ml
002 044 005Rabbit Complement5 x 1 ml
002 044 005LLyophilized Rabbit Complement5 x 1 ml
002 045 001HLA Control positive0.5 ml
002 045 002HLA Control negative0.5 ml
002 047 500Lymphofix500 ml

Do you have any questions?

We will be happy to help you!
Call us: +49 6173 6079 30

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